The haematological changes were unspecific to infection types and, showed no significant deviation between haemoparasite positive and negative animals. Sequencing of Hemoplasma positive samples identified Mycoplasma ovis, Mycoplasma wenyonii and Pseudomonas fluorescens. Sequencing and phylogenetic analyses of some PCR products identified samples with high homology with Trypanosoma brucei ovis in the A/E group while the B/T were highly related to Theileria velifera. A total of 34.1%, 23% and, 51.6% samples were positive for B/T, A/E and, Hemoplasma, respectively. The haemoparasites detected by LM were Borrelia spp, Hemoplasma spp, Babesia spp, Trypanosoma brucei spp and, Trypanosoma brucei. Comparison of LM and PCR analyses showed approximately 50% misdiagnosis of Hemoplasma spp by LM with 82.7% and 100% false negative for A/E and B/T recorded by LM, respectively. A total of 150 animals were tested by both methods with 62.7% overall infection proportion. The PCR detection rate was significantly and more than 4-fold higher compared to LM (61.5% and 14.5%, respectively P < 0.0001) alone. Relationship between the haematological changes and PCR results was evaluated.
Among them, 126 blood samples were randomly selected and PCR tested for the 18S rRNA gene of Babesia/Theileria spp (B/T) and the 16S rRNA gene of Trypanosoma brucei/Ehrlichia spp (A/E), while 91 samples were evaluated for the 16S RNA of To-Go TM ( Hemoplasma). Using LM, blood samples from a total of 173 goats in Ibadan were screened for haemoparasites and haemocytopathological evaluation.
BLOODBORNE CC FOR THE SIMS 4 UPDATE
Hence there is a need to have an update assessment of haemoparasitism of goat in Nigeria using molecular investigation. In Nigeria, most laboratories utilize only Light Microscopy (LM) for their diagnosis. Haemoparasitisms in animals are known to impose substantial economic burdens on owners.
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